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xbai hindiii digested pbad24 vector  (New England Biolabs)


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    New England Biolabs xbai hindiii digested pbad24 vector
    Overexpression of T1F reduces STm susceptibility to Sal4-mediated agglutination in the snow globe assay. ( A ) Recovered CFU/mL of STm WT + <t>pBAD24-EV</t> (empty vector; EV), Δ fimW + EV, and Δ fimW + pBAD24- fimW (pFimW) cultures in the snow globe assay. ( B ) Quantification of mannose-sensitive yeast agglutination of the STm WT + EV, Δ fimW + EV, and Δ fimW + pFimW strains. ( C ) Recovered CFU/mL of STm WT + EV, Δ fimW + EV, Δ fimA + EV, and Δ fimA + pFimW cultures in the snow globe assay. ( D ) Quantification of mannose-sensitive yeast agglutination of WT + EV, Δ fimW + EV, Δ fimA + EV, and Δ fimA + pFimW strains. For panels A and C, the indicated strains were grown to mid-log phase in the presence of 0.02% arabinose, washed in PBS, and either left untreated (circles) or treated with 15 μg/mL of Sal4 IgA (squares). After 2 h of treatment, the top of the supernatant was collected and plated on LB agar to measure CFU. For panels B and D, the indicated strains were incubated statically for 48 h in LB containing 0.02% arabinose at 37°C prior to centrifugation and resuspension in LB. Cultures were mixed with 10mg/mL yeast in the presence (triangles) and absence (hexagons) of 3% mannose in a 12-well plate, and the optical density of the wells at 600nm (OD 600 ) was measured via spectrophotometry. The strains used are SL257, SL253, SL255, SL289, and SL291. For all panels, data were obtained from three biological replicates with error bars representing the standard deviation of the mean. Statistical significance was determined by two-way ANOVA followed by Tukey’s post hoc multiple comparisons test. Asterisks (**, ***, ****) indicate P < 0.01, P < 0.001, and P < 0.0001, respectively, and ns = not significant.
    Xbai Hindiii Digested Pbad24 Vector, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 10971 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/xbai+hindiii+digested+pbad24+vector/HindIII/pmc12070745-230-8-17
    Average 99 stars, based on 10971 article reviews
    xbai hindiii digested pbad24 vector - by Bioz Stars, 2026-10
    99/100 stars

    Images

    1) Product Images from "A Salmonella enterica serovar Typhimurium genome-wide CRISPRi screen reveals a role for type 1 fimbriae in evasion of antibody-mediated agglutination"

    Article Title: A Salmonella enterica serovar Typhimurium genome-wide CRISPRi screen reveals a role for type 1 fimbriae in evasion of antibody-mediated agglutination

    Journal: Infection and Immunity

    doi: 10.1128/iai.00574-24

    Overexpression of T1F reduces STm susceptibility to Sal4-mediated agglutination in the snow globe assay. ( A ) Recovered CFU/mL of STm WT + pBAD24-EV (empty vector; EV), Δ fimW + EV, and Δ fimW + pBAD24- fimW (pFimW) cultures in the snow globe assay. ( B ) Quantification of mannose-sensitive yeast agglutination of the STm WT + EV, Δ fimW + EV, and Δ fimW + pFimW strains. ( C ) Recovered CFU/mL of STm WT + EV, Δ fimW + EV, Δ fimA + EV, and Δ fimA + pFimW cultures in the snow globe assay. ( D ) Quantification of mannose-sensitive yeast agglutination of WT + EV, Δ fimW + EV, Δ fimA + EV, and Δ fimA + pFimW strains. For panels A and C, the indicated strains were grown to mid-log phase in the presence of 0.02% arabinose, washed in PBS, and either left untreated (circles) or treated with 15 μg/mL of Sal4 IgA (squares). After 2 h of treatment, the top of the supernatant was collected and plated on LB agar to measure CFU. For panels B and D, the indicated strains were incubated statically for 48 h in LB containing 0.02% arabinose at 37°C prior to centrifugation and resuspension in LB. Cultures were mixed with 10mg/mL yeast in the presence (triangles) and absence (hexagons) of 3% mannose in a 12-well plate, and the optical density of the wells at 600nm (OD 600 ) was measured via spectrophotometry. The strains used are SL257, SL253, SL255, SL289, and SL291. For all panels, data were obtained from three biological replicates with error bars representing the standard deviation of the mean. Statistical significance was determined by two-way ANOVA followed by Tukey’s post hoc multiple comparisons test. Asterisks (**, ***, ****) indicate P < 0.01, P < 0.001, and P < 0.0001, respectively, and ns = not significant.
    Figure Legend Snippet: Overexpression of T1F reduces STm susceptibility to Sal4-mediated agglutination in the snow globe assay. ( A ) Recovered CFU/mL of STm WT + pBAD24-EV (empty vector; EV), Δ fimW + EV, and Δ fimW + pBAD24- fimW (pFimW) cultures in the snow globe assay. ( B ) Quantification of mannose-sensitive yeast agglutination of the STm WT + EV, Δ fimW + EV, and Δ fimW + pFimW strains. ( C ) Recovered CFU/mL of STm WT + EV, Δ fimW + EV, Δ fimA + EV, and Δ fimA + pFimW cultures in the snow globe assay. ( D ) Quantification of mannose-sensitive yeast agglutination of WT + EV, Δ fimW + EV, Δ fimA + EV, and Δ fimA + pFimW strains. For panels A and C, the indicated strains were grown to mid-log phase in the presence of 0.02% arabinose, washed in PBS, and either left untreated (circles) or treated with 15 μg/mL of Sal4 IgA (squares). After 2 h of treatment, the top of the supernatant was collected and plated on LB agar to measure CFU. For panels B and D, the indicated strains were incubated statically for 48 h in LB containing 0.02% arabinose at 37°C prior to centrifugation and resuspension in LB. Cultures were mixed with 10mg/mL yeast in the presence (triangles) and absence (hexagons) of 3% mannose in a 12-well plate, and the optical density of the wells at 600nm (OD 600 ) was measured via spectrophotometry. The strains used are SL257, SL253, SL255, SL289, and SL291. For all panels, data were obtained from three biological replicates with error bars representing the standard deviation of the mean. Statistical significance was determined by two-way ANOVA followed by Tukey’s post hoc multiple comparisons test. Asterisks (**, ***, ****) indicate P < 0.01, P < 0.001, and P < 0.0001, respectively, and ns = not significant.

    Techniques Used: Over Expression, Agglutination, Plasmid Preparation, Incubation, Centrifugation, Spectrophotometry, Standard Deviation

    Related Articles

    Clone Assay:

    Article Title: A Salmonella enterica serovar Typhimurium Genome-wide CRISPRi Screen Reveals a Role for Type 1 Fimbriae in Evasion of Antibody-Mediated Agglutination
    Article Snippet: .. The resulting gene fragments were cloned into a XbaI/HindIII-digested pBAD24 vector ( ) using T4 DNA ligase (NEB, Ipswich, MA). .. The resulting plasmids were cloned into competent E. coli DH5α F’ I q cells and transformants were selected on LB agar plates containing carbenicillin (NEB).

    Article Title: A Salmonella enterica serovar Typhimurium genome-wide CRISPRi screen reveals a role for type 1 fimbriae in evasion of antibody-mediated agglutination.
    Article Snippet: .. The resulting gene fragments were cloned into a XbaI/HindIII-digested pBAD24 vector (67) using T4 DNA ligase (NEB, Ipswich, MA). .. The resulting plasmids were cloned into competent E. coli DH5α F'Iq cells, and transformants were selected on LB agar plates containing carbenicillin (NEB).

    Article Title: A Salmonella enterica serovar Typhimurium genome-wide CRISPRi screen reveals a role for type 1 fimbriae in evasion of antibody-mediated agglutination
    Article Snippet: .. The resulting gene fragments were cloned into a XbaI/HindIII-digested pBAD24 vector ( ) using T4 DNA ligase (NEB, Ipswich, MA). .. The resulting plasmids were cloned into competent E. coli DH5α F' I q cells, and transformants were selected on LB agar plates containing carbenicillin (NEB).



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    xbai hindiii digested pbad24 vector  (New England Biolabs)
    99
    New England Biolabs xbai hindiii digested pbad24 vector
    Overexpression of T1F reduces STm susceptibility to Sal4-mediated agglutination in the snow globe assay. ( A ) Recovered CFU/mL of STm WT + <t>pBAD24-EV</t> (empty vector; EV), Δ fimW + EV, and Δ fimW + pBAD24- fimW (pFimW) cultures in the snow globe assay. ( B ) Quantification of mannose-sensitive yeast agglutination of the STm WT + EV, Δ fimW + EV, and Δ fimW + pFimW strains. ( C ) Recovered CFU/mL of STm WT + EV, Δ fimW + EV, Δ fimA + EV, and Δ fimA + pFimW cultures in the snow globe assay. ( D ) Quantification of mannose-sensitive yeast agglutination of WT + EV, Δ fimW + EV, Δ fimA + EV, and Δ fimA + pFimW strains. For panels A and C, the indicated strains were grown to mid-log phase in the presence of 0.02% arabinose, washed in PBS, and either left untreated (circles) or treated with 15 μg/mL of Sal4 IgA (squares). After 2 h of treatment, the top of the supernatant was collected and plated on LB agar to measure CFU. For panels B and D, the indicated strains were incubated statically for 48 h in LB containing 0.02% arabinose at 37°C prior to centrifugation and resuspension in LB. Cultures were mixed with 10mg/mL yeast in the presence (triangles) and absence (hexagons) of 3% mannose in a 12-well plate, and the optical density of the wells at 600nm (OD 600 ) was measured via spectrophotometry. The strains used are SL257, SL253, SL255, SL289, and SL291. For all panels, data were obtained from three biological replicates with error bars representing the standard deviation of the mean. Statistical significance was determined by two-way ANOVA followed by Tukey’s post hoc multiple comparisons test. Asterisks (**, ***, ****) indicate P < 0.01, P < 0.001, and P < 0.0001, respectively, and ns = not significant.
    Xbai Hindiii Digested Pbad24 Vector, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/xbai+hindiii+digested+pbad24+vector/HindIII/pmc12070745-230-8-17
    Average 99 stars, based on 1 article reviews
    xbai hindiii digested pbad24 vector - by Bioz Stars, 2026-10
    99/100 stars
    		  Buy from Supplier

    Image Search Results


    Overexpression of T1F reduces STm susceptibility to Sal4-mediated agglutination in the snow globe assay. ( A ) Recovered CFU/mL of STm WT + pBAD24-EV (empty vector; EV), Δ fimW + EV, and Δ fimW + pBAD24- fimW (pFimW) cultures in the snow globe assay. ( B ) Quantification of mannose-sensitive yeast agglutination of the STm WT + EV, Δ fimW + EV, and Δ fimW + pFimW strains. ( C ) Recovered CFU/mL of STm WT + EV, Δ fimW + EV, Δ fimA + EV, and Δ fimA + pFimW cultures in the snow globe assay. ( D ) Quantification of mannose-sensitive yeast agglutination of WT + EV, Δ fimW + EV, Δ fimA + EV, and Δ fimA + pFimW strains. For panels A and C, the indicated strains were grown to mid-log phase in the presence of 0.02% arabinose, washed in PBS, and either left untreated (circles) or treated with 15 μg/mL of Sal4 IgA (squares). After 2 h of treatment, the top of the supernatant was collected and plated on LB agar to measure CFU. For panels B and D, the indicated strains were incubated statically for 48 h in LB containing 0.02% arabinose at 37°C prior to centrifugation and resuspension in LB. Cultures were mixed with 10mg/mL yeast in the presence (triangles) and absence (hexagons) of 3% mannose in a 12-well plate, and the optical density of the wells at 600nm (OD 600 ) was measured via spectrophotometry. The strains used are SL257, SL253, SL255, SL289, and SL291. For all panels, data were obtained from three biological replicates with error bars representing the standard deviation of the mean. Statistical significance was determined by two-way ANOVA followed by Tukey’s post hoc multiple comparisons test. Asterisks (**, ***, ****) indicate P < 0.01, P < 0.001, and P < 0.0001, respectively, and ns = not significant.

    Journal: Infection and Immunity

    Article Title: A Salmonella enterica serovar Typhimurium genome-wide CRISPRi screen reveals a role for type 1 fimbriae in evasion of antibody-mediated agglutination

    doi: 10.1128/iai.00574-24

    Figure Lengend Snippet: Overexpression of T1F reduces STm susceptibility to Sal4-mediated agglutination in the snow globe assay. ( A ) Recovered CFU/mL of STm WT + pBAD24-EV (empty vector; EV), Δ fimW + EV, and Δ fimW + pBAD24- fimW (pFimW) cultures in the snow globe assay. ( B ) Quantification of mannose-sensitive yeast agglutination of the STm WT + EV, Δ fimW + EV, and Δ fimW + pFimW strains. ( C ) Recovered CFU/mL of STm WT + EV, Δ fimW + EV, Δ fimA + EV, and Δ fimA + pFimW cultures in the snow globe assay. ( D ) Quantification of mannose-sensitive yeast agglutination of WT + EV, Δ fimW + EV, Δ fimA + EV, and Δ fimA + pFimW strains. For panels A and C, the indicated strains were grown to mid-log phase in the presence of 0.02% arabinose, washed in PBS, and either left untreated (circles) or treated with 15 μg/mL of Sal4 IgA (squares). After 2 h of treatment, the top of the supernatant was collected and plated on LB agar to measure CFU. For panels B and D, the indicated strains were incubated statically for 48 h in LB containing 0.02% arabinose at 37°C prior to centrifugation and resuspension in LB. Cultures were mixed with 10mg/mL yeast in the presence (triangles) and absence (hexagons) of 3% mannose in a 12-well plate, and the optical density of the wells at 600nm (OD 600 ) was measured via spectrophotometry. The strains used are SL257, SL253, SL255, SL289, and SL291. For all panels, data were obtained from three biological replicates with error bars representing the standard deviation of the mean. Statistical significance was determined by two-way ANOVA followed by Tukey’s post hoc multiple comparisons test. Asterisks (**, ***, ****) indicate P < 0.01, P < 0.001, and P < 0.0001, respectively, and ns = not significant.

    Article Snippet: The resulting gene fragments were cloned into a XbaI/HindIII-digested pBAD24 vector ( ) using T4 DNA ligase (NEB, Ipswich, MA).

    Techniques: Over Expression, Agglutination, Plasmid Preparation, Incubation, Centrifugation, Spectrophotometry, Standard Deviation